Protection of Bovine Seryl-Transfer Ribonucleic Acid (Seryl-tRNA)Synthetase from Chemical Modification by Its Substrates, and Some Kinetic Parameters

  • 橘 芳夫
    Faculty of Pharmaceutical Sciences, Nagoya City University
  • 水谷 隆治
    Faculty of Pharmaceutical Sciences, Nagoya City University

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説明

Amino acid residues contained in the recognition sites of seryl-transfer ribonucleic acid (tRNA) synthetase (SerRS) were studied by chemical modification. Ser residues were modified with phenylmethanesulfolyl fluoride, and appeared to be unnecessary for the recognition. However, the modification of Arg residues with phenylglyoxal, His residues with diethylpyrocarbonate and sulfhydryl groups with 5,5'-dithiobis(2-nitrobenzoic acid), N-ethylmaleimede, iodoacetic acid or iodoacetamide showed that these residues were essential for the tRNA recognition by SerRS.Protection experiments with substrates from inactivation of Ser-tRNA formation by modification suggested that Arg residues interact with the γ-phosphate of adenosine triphosphate and tRNA.Modification of sulfhydryl groups showed that the groups interact with the hydroxyl groups of ribose of the CCA-end on tRNA. Furthermore, in order to understand the recognition mechanism between SerRS and tRNA^<Ser>, some kinetic parameters such as the K_m and V_<max> values of yeast tRNA^<Ser> and E. coli tRNA^<Ser> for bovine SerRS were compared with the values of bovine tRNA^<Ser>.

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詳細情報 詳細情報について

  • CRID
    1573668927250795776
  • NII論文ID
    110003626751
  • NII書誌ID
    AA00602100
  • ISSN
    00092363
  • 本文言語コード
    en
  • データソース種別
    • CiNii Articles

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