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Multi-locus genotyping of bottom fermenting yeasts by single nucleotide polymorphisms indicative of brewing characteristics

  • IKUSHIMA Shigehito
    Central Laboratories for Frontier Technology, KIRIN Holdings Company, Ltd.
  • TATEISHI Yoshiyuki
    Brewing Technology Development Center, Kirin Brewery Company, Ltd.
  • KANAI Keiko
    Central Laboratories for Frontier Technology, KIRIN Holdings Company, Ltd.
  • SHIMADA Emiko
    Central Laboratories for Frontier Technology, KIRIN Holdings Company, Ltd.
  • TANAKA Misa
    Brewing Technology Development Center, Kirin Brewery Company, Ltd.
  • ISHIGURO Tatsuji
    Central Laboratories for Frontier Technology, KIRIN Holdings Company, Ltd.
  • MIZUTANI Satoru
    Central Laboratories for Frontier Technology, KIRIN Holdings Company, Ltd.
  • KOBAYASHI Osamu
    Central Laboratories for Frontier Technology, KIRIN Holdings Company, Ltd.

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Abstract

Yeast plays a capital role in brewing fermentation and has a direct impact on flavor and aroma. For the evaluation of competent brewing strains during quality control or development of novel strains it is standard practice to perform fermentation tests, which are costly and time-consuming. Here, we have categorized DNA markers which enable to distinguish and to screen brewing strains more efficiently than ever before. Sequence analysis at 289 loci in the genomes of six bottom fermenting Saccharomyces pastorianus strains revealed that 30 loci contained single nucleotide polymorphisms (SNPs). By determining the nucleotide sequences at the SNP-loci in 26 other S. pastorianus strains and 20 strains of the top fermenting yeast Saccharomyces cerevisiae, almost all these strains could be discriminated solely on the basis of the SNPs. By comparing the fermentative phenotypes of these strains we found that some DNA markers showed a strong association with brewing characteristics, such as the production of ethyl acetate and hydrogen sulphide (H_2S). Therefore, the DNA markers we identified will facilitate quality control and the efficient development of brewing yeast strains.

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