Biochemical Studies on Streptolysin S Formation:III. Intracellular Streptolysins

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  • TAKETO AKIRA
    Departments of Biochemistry and Pharmacology, School of Medicine, Kanazawa University
  • TAKETO YORIKO
    Departments of Biochemistry and Pharmacology, School of Medicine, Kanazawa University

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1. Separation of intracellular streptolysin S (ICH-S) and intracellular streptolysin O (ICH-O) contained in the streptococcal extract were achieved by gel filtration through Sephadex G-200.<br> 2. ICH-S was a large complex nondif-fusible into the dextran gel and appeared in earlier fractions, while extracellular SRNA-SL was retarded and appeared in the rear frac-tions. ICH-O was similar to extracellular streptolysin O in respect of biological proper-ties as well as of the gel filtration behaviours.<br> 3. The previous conclusion that RNA-SLS was formed by transferring the hemolytic component of ICH-S to AF, was reconfirmed by the gel filtration experiments.<br> The authors wish to express their appreciation to Profs. H. Okamoto and S. Kuno for their encourage-ment and for the criticism of the manuscript.

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