Quantitative analysis of affinity chromatography of trypsin. A new technique for investigation of protein-ligand interaction.:A New Technique for Investigation of Protein-ligand Interaction

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  • KASAI Ken-ichi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University
  • ISHII Shin-ichi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University

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説明

A new method for quantitative analysis of affinity chromatography which allows estimation of dissociation constants of a protein-ligand (either soluble or immobilized) complex is proposed. This method was successfully applied to a trypsin-glycylgly-cyl-L-arginine agarose system.

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