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Studies on the Amphoteric Properties of Taka-amylase A:II. Unmasking of Acid Binding Sites
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- TAKAGI TOSHIO
- the Department of Biology, Faculty of Science and the Division of Physical Chemistry, Institute for Protein Research, Osaka University
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- ISEMURA TOSHIZO
- the Department of Biology, Faculty of Science and the Division of Physical Chemistry, Institute for Protein Research, Osaka University
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Description
1. The ionization behavior of Taka-amy-lase A(TAA) in the acid pH region was studied by pH titration. It was found that more than 20 of the 40 carboxyl groups in a TAA molecule are in a masked state as carboxylate ions and combine with hydrogen ions irreversibly below pH 4.3.<br> 2. From measurement of the optical rotation, it was found that the acid-denaturation of TAA occurs simultaneously with the unmasking of the carboxylate groups.<br> 3. These results, together with the previous observations on the abnormal ionization of phenolic hydroxyl groups in TAA, suggest that carboxylate-phenolic hydroxyl hydrogen bonds play a part in maintaining the stable structure of TAA.<br> The authors wish to thank Sankyo Co., Ltd. for a supply of "Takadiastase Sankyo". The authors are also grateful to the Ministry of Education for a grant covering part of the expenses.
Journal
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- J Biochem (Tokyo)
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J Biochem (Tokyo) 49 (1), 43-48, 1961
The Japanese Biochemical Society
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Details 詳細情報について
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- CRID
- 1573668927917083904
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- NII Article ID
- 130003536594
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- ISSN
- 0021924X
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- Text Lang
- en
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- Data Source
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- CiNii Articles