Fractionation of Transfer Ribonucleic Acids from <i>Torulopsis utilis</i>:II. Chromatography on a DEAE-Sephadex Column in Phosphate and in Borate

J-STAGE

Search this article

Description

Chromatography of Torulopsis utilis tRNA on DEAE-Sephadex columns with various concentrations of phosphate or of borate were reported.<br> Of the solvents tested, M phosphate-5% DMFA and 0.5M borate were the most suit-able for fractionation of tRNA, since they gave good purification and recovery of the various amino acid specific tRNA's. The distribution patterns of the various acceptor activities dif-fered in the M phosphate-DMFA, 0.5M borate, ammonium sulfate-DMFA and DMFA-KGI systems.<br> Several amino acid-acceptor activities were resolved into multiple peaks especially with the 0.5M borate system.<br> It was necessary to control the temperature to obtain reproducible elution patterns on chromatography with phosphate or borate, unlike the case with ammonium sulfate.<br> The expences of this study were defrayed in part by a grant from the Ministry of Education.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 60 (5), 526-532, 1966

    The Japanese Biochemical Society

Details 詳細情報について

Report a problem

Back to top