Studies on Phosphoproteins from Fish Eggs:I. Isolation and Characterization of a Phosphoprotein from the Eggs of Pacific Herring

J-STAGE
  • INOUE Sadako
    The Department of Biophysics and Biochemistry, Faculty of Science, the University of Tokyo
  • KANEDA-HAYASHI Tomoko
    The Department of Biophysics and Biochemistry, Faculty of Science, the University of Tokyo
  • SUGIYAMA Hiroyuki
    The Department of Biophysics and Biochemistry, Faculty of Science, the University of Tokyo
  • ANDO Toshio
    The Department of Biophysics and Biochemistry, Faculty of Science, the University of Tokyo

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説明

1. A phosphoprotein containing 10.6% of phosphorus has been isolated from the eggs of Pacific herring by means of phenol extraction and DEAE-cellulose column chromatography.<br> 2. Only 8 kinds of amino acid including a small amount each of the 2 kinds were detectable in the acid hydrolysate of the phosphoprotein. The protein contained only serine as hydroxyl group-bearing amino acid and this amino acid occupied more than 2/3 of total amino acid residues. No amino sugar was detectable.<br> 3. A molecular weight of 4, 200±400 was calculated from the sedimentation equilibrium data for the phosphoprotein. The following formula has been given tentatively for the protein on the basis of the molecular weight: (Lys)1(Arg)1(Asp)1(Phe)1 (Leu)3(Ser)23-36(Phosphate)16.<br> 4. The results of an alkali-catalyzed β-elimination reaction suggested that all the phosphorus in the phosphoprotein are present in the form of monoesters linked to the hydroxyl groups of serine.<br> 5. Two moles of DNP-group were introduced per 4, 200g of the protein. The Nterminal residue of the major component of the preparation was determined to be lysine by the analysis of DNP-products.

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