Enzymatic Formation of Xanthurenic Acid 8-Methyl Ether, an Endogenous Carcinogen, in Animal Tissues
-
- SUZUKI MAKIKO
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University
-
- TABARA MASAYOSHI
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University
-
- IINUMA FUMIO
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University
-
- WATANABE MITSUO
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University
Search this article
Abstract
An enzyme activity forming xanthurenic acid 8-methyl ether, a carcinogenic tryptophan metabolite, was found in the organs of various animals by using a new method of analysis. After incubation of a 105000g supernatant of the tissues with xanthurenic acid and S-adenosylmethionine at pH 10.0,the xanthurenic acid 8-methyl ether was separated by high-performance liquid chromatography on a LiChrosorb RP-18 column and determined fluorometrically. Potent enzyme activity was found in the liver and kidney of pig and wild boar, and in bovine liver. Low activity was found in the liver and kidney of sheep, deer, and monkey. Our data suggest that the ether may be formed from xanthurenic acid by this enzyme, because the enzyme activity was high in porcine liver, and pigs excrete xanthurenic acid 8-methyl ether in the urine.
Journal
-
- Chemical & pharmaceutical bulletin
-
Chemical & pharmaceutical bulletin 32 (6), 2340-2345, 1984-06-25
The Pharmaceutical Society of Japan
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1574231877177464064
-
- NII Article ID
- 110003663600
-
- NII Book ID
- AA00602100
-
- ISSN
- 00092363
-
- Text Lang
- en
-
- Data Source
-
- CiNii Articles