Characterization of N-glycolylneuraminic acid-containing glycosphingolipids from a Marek's disease lymphoma-derived chicken cell line, MSB1, as tumor-associated heterophile Hanganutziu-Deicher antigens.

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  • HIGASHI Hideyoshi
    Department of Pathology, Research Institute for Microbial Diseases, Osaka University
  • IKUTA Kazuyoshi
    Department of Pathology, Research Institute for Microbial Diseases, Osaka University
  • UEDA Shigeharu
    Department of Pathology, Research Institute for Microbial Diseases, Osaka University
  • KATO Shiro
    Department of Pathology, Research Institute for Microbial Diseases, Osaka University
  • HIRABAYASHI Yoshio
    Department of Biochemistry, Shizuoka College of Pharmacy, Oshika, Shizuoka
  • MATSUMOTO Makoto
    Department of Biochemistry, Shizuoka College of Pharmacy, Oshika, Shizuoka
  • NAIKI Masaharu
    Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University

説明

Heterophile, Hanganutziu-Deicher (HD) antigen-active N-glycolylneuraminic acidcontaining glycosphingolipids (GSLs) were detected as tumor-associated foreign antigens of a Marek's disease lymphoma-derived cell line, MSB 1, by enzyme-immunoassay with chicken antibody against N-glycolylneuraminyl-lactosylceramide (anti-NeuGc-LacCer). At least three species of HD antigen-active GSLs were detected by two-dimensional thin-layer chromatography (TLC) combined with enzyme-immunoassay. The reactivities of the GSLs with anti-NeuGc-LacCer, their behaviors on two-dimensional TLC and the results of an endo-β-galactosidase digestion study indicated that these three GSLs were NeuGc-LacCer (NeuGcα2-3Galβ1-4Glc-Cer), NeuGc-nLcOse4Cer (NeuGcα2-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc-Cer) and NeuGc-nLcOse6Cer (NeuGcα2-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc-Cer).

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