Ergosterol biosynthesis in yeast. Pathways in the late stages and their variation under various conditions.:Pathways in the Late Stages and Their Variation under Various Conditions

J-STAGE
  • OSUMI Takashi
    Department of Chemistry, Faculty of Scienec, Kyoto University
  • TAKETANI Shigeru
    Department of Chemistry, Faculty of Scienec, Kyoto University
  • KATSUKI Irohiko
    Department of Chemistry, Faculty of Scienec, Kyoto University
  • KUHARA Tomiko
    Research Laboratory of Gas Chromatography-Mass Spectromery, Kurume Uiversity, School of Medicine
  • MATSUMOTO Isamu
    Research Laboratory of Gas Chromatography-Mass Spectromery, Kurume Uiversity, School of Medicine

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説明

[Methyl-14C] methionine was supplied to yeast cells under aerobic and anaerobic conditions for the investigation of the pathway for ergosterol biosynthesis after the methylation of the side-chain. Under aerobic conditions, the incorporation of radioactivity into ergosterol was high. With a limited oxygen supply, in contrast, the radioactivity was first accumulated in ergosta-7, 24 (28)-dien-3β-ol and ergosta-8, 24 (28)-dien-3β-ol, and then transferred to ergost-7-en-3β-ol, ergost-8-en-3β-ol and ergosta-7, 22-dien-3β-ol with time. Under strictly anaerobic conditions, a double bond was introduced neither to Δ5 nor to Δ22. The results of the tracer experiments suggested the operation of several pathways in the late stages of ergosterol biosynthesis. It was also suggested that the main pathways varied depending on the conditions such as oxygen supply and other factors. The above conclusion was supported by the results of the analyses of the sterol compositions of the cells grown under various conditions.

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