Autophosphorylation of Calmodulin-Dependent Protein Kinase IV from Rat Cerebral Cortex.
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- Kameshita Isamu
- Department of Biochemistry, Asahikawa Medical College
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- Fujisawa Hitoshi
- Department of Biochemistry, Asahikawa Medical College
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説明
Calmodulin-dependent protein kinase IV from rat cerebral cortex undergoes autophosphorylation in response to Ca2+ and calmodulin, resulting in its marked enzymatic activation. Autophosphorylation occurred at several sites on CaM-kinase IV, depending upon the enzyme concentration. Among them, Ser437 was almost exclusively phosphorylated at enzyme concentrations lower than 10 μg/ml, and autophosphorylation at Ser437 was responsible for marked activation of the enzyme through decreases in the Km values for its substrates and an increase in the Vmax value. The Ca2+/calmodulin-independent activity of CaM-kinase IV was also markedly stimulated by autophosphorylation, but even after autophosphorylation it amounted only about 17% of the total enzyme activity detected in the presence of Ca2+/calmodulin.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 113 (5), 583-590, 1993
The Japanese Biochemical Society
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詳細情報 詳細情報について
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- CRID
- 1574231877870011520
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- NII論文ID
- 130003531866
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- ISSN
- 0021924X
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles