Studies on the Substrate Specificity of Taka-amylase A:VII. Synthesis of Some Analogues of Phenyl α-Maltoside and Enzymatic Investigation

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Three analogues of phenyl α-maltoside which is a long known synthetic substrate of Taka-amylase A [EC 3. 2. 1. 1] were synthesized. Two of the new compounds, phenyl 2-O-methyl-α-maltoside and phenyl 2-deoxy-α-maltoside were found to be not susceptible to the enzyme. They showed considerable inhibitory action against the enzyme when phenyl α-maltoside was used as a substrate. One of the new compounds, phenyl 4-O-α-glucosyl-α-mannoside, was also found to be immune to the enzyme. Rather amazingly, the last compound showed no inhibitory action against the enzyme.

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