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Mapping phospho-catalytic dependencies of therapy-resistant tumours reveals actionable vulnerabilities
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Description
Phosphorylation networks intimately regulate mechanisms of response to therapies. Mapping the phospho-catalytic profile of kinases in cells or tissues remains a challenge. Here, we introduce a practical high-throughput system to measure the enzymatic activity of kinases using biological peptide targets as phospho-sensors to reveal kinase dependencies in tumour biopsies and cell lines. A 228-peptide screen was developed to detect the activity of60 kinases, including ABLs, AKTs, CDKs and MAPKs. Focusing on BRAF
Journal
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- Nature Cell Biology
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Nature Cell Biology 21 778-790, 2019-06-01
Springer Science and Business Media LLC
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Keywords
- Adult
- Male
- 571
- Indoles
- Protein Kinase C-alpha
- MAP Kinase Signaling System
- Drug Resistance
- Kaplan-Meier Estimate
- Medical and Health Sciences
- Cell Line
- 3-Phosphoinositide-Dependent Protein Kinases
- Proto-Oncogene Proteins c-pim-1
- Cell Line, Tumor
- 2.1 Biological and endogenous factors
- Humans
- Aetiology
- Phosphorylation
- Melanoma
- Protein Kinase Inhibitors
- Cancer
- Aged
- Neoplastic
- Sulfonamides
- Tumor
- Biological Sciences
- Middle Aged
- Gene Expression Regulation, Neoplastic
- Good Health and Well Being
- Gene Expression Regulation
- 5.1 Pharmaceuticals
- Drug Resistance, Neoplasm
- Biochemistry and cell biology
- Neoplasm
- Female
- Biochemistry and Cell Biology
- Development of treatments and therapeutic interventions
- Colorectal Neoplasms
- Peptides
- Developmental Biology
Details 詳細情報について
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- CRID
- 1870020692543994624
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- ISSN
- 14764679
- 14657392
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- PubMed
- 31160710
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- Data Source
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- OpenAIRE