Mapping phospho-catalytic dependencies of therapy-resistant tumours reveals actionable vulnerabilities

DOI PubMed Open Access

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Phosphorylation networks intimately regulate mechanisms of response to therapies. Mapping the phospho-catalytic profile of kinases in cells or tissues remains a challenge. Here, we introduce a practical high-throughput system to measure the enzymatic activity of kinases using biological peptide targets as phospho-sensors to reveal kinase dependencies in tumour biopsies and cell lines. A 228-peptide screen was developed to detect the activity of60 kinases, including ABLs, AKTs, CDKs and MAPKs. Focusing on BRAF

Journal

  • Nature Cell Biology

    Nature Cell Biology 21 778-790, 2019-06-01

    Springer Science and Business Media LLC

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