[8] Use of transgenic mice to eliminate retinoic acid receptor function in specific tissues
説明
Publisher Summary This chapter proposes a novel strategy to examine the physiological functions of retinoic acid (RA) in a tissue-specific manner. This novel strategy has revealed the absolute requirement of RA in normal skin development. In addition to the epidermis, there are several other interesting targets in which to express the dominant-negative receptor for RA (RAR), that is, lung, liver, intestine, bone, and testis. Thus, it would be possible to dissect the roles of RA systemically in these organs during embryogenesis and in adults. Because the structure and mode of actions of the thyroid hormone receptor (TR)/RAR subfamily are quite homologous, it is possible to construct a dominant-negative molecule of other members by the same procedure, which might be used to discover unknown physiologic functions of other lipophilic molecules. An application of this strategy to so-called orphan receptors would be quite difficult and might reveal the function of the orphan receptors before their ligands are known.
- Tweet
キーワード
- Cell Nucleus
- Receptors, Thyroid Hormone
- Sequence Homology, Amino Acid
- Transcription, Genetic
- Receptors, Retinoic Acid
- Molecular Sequence Data
- Chromosome Mapping
- Mice, Transgenic
- Oncogene Proteins v-erbA
- Transfection
- Recombinant Proteins
- Cell Line
- Mice
- Phenotype
- Animals
- Humans
- Amino Acid Sequence
- Sequence Alignment
- Conserved Sequence