The formation of “ribosomal RNA” in escherichia coli during recovery from magnesium starvation

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Abstract 1. 1. When Escherichia coli cells are incubated in a medium lacking magnesium, they lose most of their ribosomes without loss of viability, as reported by McCarthy . 2. 2. Upon the addition of magnesium, the cells accumulate “nascent ribosomal RNA” in the particles which appear to correspond to those synthesized in the presence of chloramphenicol. 3. 3. The nascent ribosomal RNA has a slightly different sedimentation coefficient from that of the mature ribosomal RNA. This is probably due to the difference in secondary structure between these two types of RNA, a difference which can be shown by the hyperchromicity of the nascent form upon heating.

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