Uptake of O-Alkyl Erythromycin Derivatives in the Lung Tissue and Cells of Rats

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To determine the in vivo uptake by the rat lung, each derivative was injected into the external jugular vein. Five seconds after the injection, the lung was removed and homogenized. The supernatant obtained by centrifugation of the homogenate at 3000 rpm (2,000×g) for 10 min was used for the microbiological assay. The assay was performed by the paper disc method, using Micrococcus luteus ATCC 9341 as the test organism

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