Chromosome and Plasmid Partition in Escherichia coli

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INTRODUCTION 283 ANALYSIS OF GENES INVOLVED IN CHROMOSOME PARTITION 286 Two Categories of Mechanisms.. . . . . . . . .. . . . . . ....... . . . . ... . . . . . . . . . . . . . ..... . . . . . . 286 Bacterial par Mutants Defective in Topoisomerases .... 286 muk Mutants; Mutants that Produce Chromosome-less Cells......... ........ 288 THE mukB GENE AND ITS PRODUCT 290 Properties of the mukB Mutants..... ..... ........ 290 Predicted Secondary Structure of MukB Protein....... .. . . . . . . . ........ . . . . 291 Properties of MukB Protein in vitro. ...... . . .... . . . . . .... . . . .. . . .. . . . . . . . . . . . . . . . . 293 Filamentous Protein Polymers in E. coli 294 PARTITION MECHANISM OF PLASMlDS 295 Plasmid-Encoded Proteins and the cis-Acting Region of F and PI Plasm ids Essential for Partition 295 Partition Genes of the lncFfl Plasmids Rl and NRI . .... . . . . . . . . . .. 301 The cis-Acting par Site of pSCIOI Plasmid 302 Partition of a Mini-F Plasmid in a mukB-Disrupted Null Mutant 302 CONCLUDING REMARKS ....... ........ . 303

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