Purification and characterization of arylsulfatase from sea urchin (Hemicentrotus pulcherrimus) embryos

Hiroshi Sasaki, Koji Akasaka, Tsugio Shiroya, Hiraku Shimada

doi:10.1016/0305-0491(87)90093-9

Description

Abstract 1. 1. Arylsulfatase was extracted from sea urchin (Hemicentrotus pulcherrimus) plutei and purified to electrophoretical homogeneity by means of DEAE-cellulose, acetone fractionation and Sepharose CL-6B, successively. 2. 2. The molecular weight of this enzyme was approx, 670,000. The molecular weight of a single subunit was approx. 63,000. The Km value for p-nitrophenyl sulfate was 0.59 mM. 3. 3. This enzyme was competitively inhibited by the sulfate ion and was classified as the type II arylsulfatase. The pH optimum was between 5.0 and 6.0.

Journal

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 88 147-152, 1987-01-01

Elsevier BV

Details 詳細情報について

CRID: 1870302167706657792

DOI: 10.1016/0305-0491(87)90093-9

ISSN: 03050491

Data Source

OpenAIRE

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