Free-radical mechanism by which triphenyltetrazolium chloride stimulates aerobic oxidation of NADPH by microsomes

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Abstract 1. 1. Free-radical intermediate of 2,3,5-triphenyltetrazolium chloride (TTC) (TTC • ) in the NADPH-microsomes-TTC system has been detected by means of ESR spectroscopy. 2. 2. In contrast to reduced TTC (triphenylformazan), TTC • is easily autoxidizable. The product of reduced O 2 is H 2 O 2 . 3. 3. The mechanism by which TTC stimulates the aerobic oxidation of NADPH by microsomes has been interpreted based on the above findings. 4. 4. Autoxidizability of TTC • may also shed some light on the controversies about the mechanisms of enzymatic reduction of TTC.

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