Nonlinear Deep-UV excitation microscopy for high-resolution multicolor imaging of fluorescent proteins

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Fluorescent proteins (FPs) are essential tools for studies of cellular activities and structures. Multicolor labeling with FPs allows us to visualize the relations between proteins or organelles which evoke a variety of biological functions. Although recent advances in multicolor fluorescence microscopy realized simultaneous imaging of multiple fluorescent probes, there are still issues such as chromatic aberration and difficulty in preparing a sample which provides reasonable fluorescence intensity of each fluorescent probe at a same excitation intensity.

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