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説明
Recent evidence suggests a role of reactive oxygen intermediates (ROI) in intracellular signaling and regulation of gene expression. We examined whether expression of interleukin-8 (IL-8), a key cytokine in the inflammatory responses of gastric epithelial cells, is sensitive to antioxidants and oxidative stress. IL-8 secretion was quantified by IL-8 enzyme-linked immunosorbent assay, and IL-8 mRNA expression was determined by northern blot analysis. Electrophoretic mobility shift assay was performed to detect the transcription factor, nuclear factor kappaB (NF-kappaB). N-Acetylcysteine (NAC) or dimethylsulfoxide inhibited IL-8 expression induced by tumor necrosis factor-alpha (TNF-alpha) or interleukin-1beta (IL-1beta). Externally applied H2O2 significantly up-regulated IL-8 expression. TNF-alpha-induced activation of NF-kappaB activity was suppressed by NAC, and H2O2 caused significant activation of NF-kappaB. Since ROI production is increased in the inflamed gastric mucosa, for example, in H. pylori-associated gastritis, the present results suggest that ROI may be an important modulator of IL-8 expression in gastric mucosal cells.
収録刊行物
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- Digestive diseases and sciences
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Digestive diseases and sciences 44 (2), 1999-02-01
Kluwer Academic Publishers-Plenum Publishers
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キーワード
- Electrophoresis
- Tumor Necrosis Factor-alpha
- Interleukin-8
- NF-kappa B
- Enzyme-Linked Immunosorbent Assay
- Hydrogen Peroxide
- Blotting, Northern
- Acetylcysteine
- Gastric Mucosa
- Tumor Cells, Cultured
- Humans
- Dimethyl Sulfoxide
- RNA, Messenger
- Reactive Oxygen Species
- Oxidation-Reduction
- Interleukin-1
- Transcription Factors
