Quantitation of choline glycerophospholipids that contain carboxylate residues by fluorometric high-performance liquid chromatography
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説明
Abstract A reversed-phase high-performance liquid chromatographic method was developed for the quantitation of oxidized choline glycerophospholipids (CGPs) that contain carboxylate residues, after the derivatization of these CGPs with the fluorescent probe 9-anthrydiazomethane (ADAM). The probe reacted efficiently with the carboxylate residues of the CGPs at room temperature. Fluorescent derivatives of carboxylic CGPs were well separated into glutaryl and azelaoyl residues, which are the predominant carboxylic CGP products of the oxidation of naturally occurring CGP. The relationship between the amount of each derivative and the signal was linear over a wide range and amounts as low as a few picomoles of carboxylic glycerophospholipids could be detected.
収録刊行物
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- Journal of Chromatography A
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Journal of Chromatography A 724 131-136, 1996-02-01
Elsevier BV