Production of Monoclonal Antibody Inhibiting Dipeptidy-laminopeptidase IV Activity of<i>Porphyromonas gingivalis</i>
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説明
Porphyromonas gingivalis is a Gram-negative anaerobic bacterial species implicated as an important pathogen in the development of adult periodontitis. We previously cloned a gene encoding dipeptydilaminopeptidase IV (DAPIV) from P. gingivalis. In the present study, for immunological diagnosis and development of passive immunization, we produced a mouse monoclonal antibody (MAb) capable of inhibiting the DAPIV activity of P. gingivalis using highly purified recombinant DAPIV as an immunogen. The constructed MAb, designated as MAb-Pg-DAP-1, significantly inhibited DAPIV activity in P. gingivalis, as well as slightly inhibited that in other gram-negative bacteria such as Porphyromonas endodontalis and Prevotella loesheii, whereas no inhibition was seen in the gram-positive bacteria Streptococcus mutans and Actinomyces viscosus. Furthermore, the MAb did not inhibit DAPIV enzyme activity in human serum. This novel MAb may be useful for the development of immunological diagnosis capability and in passive immunization.
収録刊行物
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- Hybridoma and Hybridomics
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Hybridoma and Hybridomics 22 147-151, 2003-06-01
Mary Ann Liebert Inc
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キーワード
- Mice, Inbred BALB C
- Sequence Homology, Amino Acid
- Dipeptidyl Peptidase 4
- Blotting, Western
- Immunoblotting
- Molecular Sequence Data
- Dose-Response Relationship, Immunologic
- Antibodies, Monoclonal
- Enzyme-Linked Immunosorbent Assay
- Recombinant Proteins
- Mice
- Gram-Negative Bacteria
- Animals
- Electrophoresis, Polyacrylamide Gel
- Protease Inhibitors
- Amino Acid Sequence
- Porphyromonas gingivalis