Hybrid with Rabbit Skeletal DTNB-Light Chains of Heavy Meromyosin, Myosin, and Glycerinated Fibers from Akazara Scallop Adductor1
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説明
It was shown in our previous report (Ojima et al. (1983) J. Biochem. 94, 307-310) that hybridization of Akazara scallop "desensitized" myosin with rabbit skeletal DTNB-light chains led to inhibition of the Mg-ATPase activity of acto-desensitized myosin but to enhancement of its superprecipitation activity. The following are now found: Development of tension in desensitized glycerinated fibers of Akazara adductor is significantly improved when DTNB-light chains are added to the fiber bath. The actin-affinity of desensitized heavy meromyosin in the presence of ATP but in the absence of Ca2+ is decreased by hybridization with chicken gizzard 20K dalton-light chains but significantly increased by that with DTNB-light chains. It is therefore suggested that the increase in actin-binding may account for the enhancing effect of DTNB-light chains on the superprecipitation and on the tension development.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 96 1109-1115, 1984-10-01
Oxford University Press (OUP)
