Characterization of a macrophage chemotactic lymphokine produced by purified protein derivative stimulation in vitro and in vivo
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説明
Abstract Using an immunoadsorbent column conjugated with anti-macrophage chemotactic factor-c (anti-MCF-c), MCF-c which has been separated and highly purified from a delayed-type hypersensitivity reaction (DHR) site, shares common antigenicity with the major macrophage chemotactic lymphokine released from purified protein derivative (PPD)-stimulated lymphocytes and also macrophage chemotactic lymphokine from phytohemagglutinin (PHA)-stimulated lymphocytes. Using a combination of the immunoadsorbent column and Sephadexgel chromatography these two lymphokines are purified to homogeneity from PPD- or PHA-stimulated guinea pig lymphocyte culture supernatants. These observations, taken in conjunction with the similarity in biological activities, physicochemical properties, and antigenicities, suggest that these two mediators are very similar, or possibly identical. MCF-c with chemotactic activity for macrophages seemed to exist as complexes with serum protein at the skin site of PPD-induced DHR in guinea pigs. The active substance, separated from the complexes under acid conditions, is indistinguishable from the major macrophage chemotactic lymphokine released by PPD stimulation with respect to antigenicity, heat stability, and non-diffusibility. They both have a molecular weight of about 12, 500. The chemotactic lymphokine formed comparable complexes with serum protein under neutral conditions; however, this complex dissociated in acid without loss of activity.
収録刊行物
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- Cellular Immunology
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Cellular Immunology 67 213-228, 1982-03-01
Elsevier BV
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キーワード
- Male
- Lymphokines
- Chemical Phenomena
- Chemotactic Factors
- Chemistry, Physical
- Macrophages
- Guinea Pigs
- Chemical Fractionation
- Lymphocyte Activation
- Tuberculin
- Chemistry
- Animals
- Electrophoresis, Polyacrylamide Gel
- Hypersensitivity, Delayed
- Phytohemagglutinins
- Immunoelectrophoresis
- Immunosorbent Techniques