Characterization of N- and C-termini of HER2 protein by mass spectrometry-based sequencing: Observation of two types of N-terminal sequence
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説明
Abstract N- and C-terminal amino acid sequences of human epidermal growth factor receptor-2 (HER2), purified from breast cancer cell lines (SK-BR-3 and BT-474), were analyzed at the protein level using mass spectrometry. Two N-terminal peptides and one C-terminal peptide were obtained. Their MALDI-PSD analyses indicated that one of the N-terminal sequences and the C-terminal sequence were identical to those found in UniProtKB/Swiss-Prot. However, the other N-terminal sequence was not in the database and was considered as a novel variant. This variant may result from the cleavage at a potential alternative site for cleavage of the signal peptide.
収録刊行物
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- International Journal of Mass Spectrometry
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International Journal of Mass Spectrometry 373 81-87, 2014-11-01
Elsevier BV