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説明
Abstract 1. 1. A thermal denaturation technique was employed to measure the changes in the proportion of weakly stabilized parts of chromatin, which presumably correspond to its template active parts. 2. 2. Comparing the chromatin of developing sea urchin embryos at successive stages, a clear distinction was found in the pattern of the denaturation profile between early and late blastula stages, suggesting that the template availability was released on a large scale at late blastula stage. 3. 3. Sperm chromatin, in which a weakly stabilized region could hardly, if at all, be recognized, was treated with heparin. It was found that heparin was able to cause destabilization in chromatin in vitro to various degrees depending on the concentration. 4. 4. Sperm chromatin was next treated with proteoglycan extracted from sea urchin embryos, which has been proposed as a putative gene activator of cytoplasmic origin. Partial destabilization of the chromatin was observed to occur, and the effect was found to be retained in chromatin after washing. 5. 5. The change of chromatin revealed by a physical assay method is discussed in relation to the mechanism of nucleocytoplasmic interactions.
収録刊行物
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- Experimental Cell Research
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Experimental Cell Research 102 153-161, 1976-10-01
Elsevier BV