Biocatalytic Preparation of Chiral Alcohols with Micro Green Algae: Bio-reduction of Carbonyl Compounds by Chlorogonium Strains as a Novel Biocatalyst

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<jats:p>Chlorogonium strains were screened to examine the potential ability of microgreen algae to act as biocatalysts. Two culture media (National Institute for Environmental Studies (NIES) recommended AF-6 medium and a synthetic AY medium) were tested for the use in the liquid culture of three algal strains. When static cultured in the AY medium containing acetate and yeast extract under continuous light conditions, these strains showed promising growth. Thus, the stereoselective reduction of various carbonyl compounds using these strains was investigated. This study discovered that these strains could reduce aliphatic and aromatic a-keto esters, a-substituted b-keto ester, and aromatic a-keto amide. Based on the conversion ratio and stereoselectivity of the yielded alcohols, C. elongatum NIES-1357 strains cultured in the AY medium is a potential biocatalyst for the stereoselective reduction of aliphatic a-keto esters, 2-chlorobenzoylformamide, and ethyl 2-methyl-3-oxobutanoate to the corresponding chiral alcohols in the presence of L-alanine.</jats:p>

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