Stringent structural plasticity of dendritic spines revealed by two-photon glutamate uncaging in adult mouse neocortex <i>in vivo</i>
説明
<jats:title>Abstract</jats:title><jats:p>Two-photon uncaging of glutamate is widely utilized to characterize structural plasticity in brain slice preparations <jats:italic>in vitro</jats:italic>. In this study, we investigated spine plasticity by using, for the first time, glutamate uncaging in the neocortex of adult mice <jats:italic>in vivo</jats:italic>. Spine enlargement was successfully induced in a smaller fraction of spines in the neocortex (22%) than in young hippocampal slices (95%), even under a low magnesium condition. Once induced, the time course and mean amplitudes of long-term enlargement were the same (81%) as those <jats:italic>in vitro</jats:italic>. However, low-frequency (1–2 Hz) glutamate uncaging caused spine shrinkage in a similar fraction (34%) of spines as <jats:italic>in vitro</jats:italic>, but spread to the neighboring spines less frequently than <jats:italic>in vitro</jats:italic>. Thus, we found that structural plasticity can occur similarly in the adult neocortex <jats:italic>in vivo</jats:italic> as in the hippocampus <jats:italic>in vitro</jats:italic>, although it happens stringently in a smaller subset of spines.</jats:p>
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詳細情報 詳細情報について
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- CRID
- 1360585256671077504
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- DOI
- 10.1101/577742
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- 資料種別
- preprint
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- データソース種別
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- Crossref
- OpenAIRE