Isolation of apolipoprotein A-I-containing lipoproteins by immunoaffinity chromatography using a formylated derivative of cellulose gel
この論文をさがす
説明
Immunoaffinity chromatography is an important tool used to purify the single component from the mixture such as plasma. In the present study, we designed a simple and efficient method for isolating lipoproteins by anti-APO A-I and Apo A-II immunoaffinity columns using Formyl-Cellufine gel. With the combination of these two columns, we quantitated two different species of Apo A-I containing lipoproteins; lipoprotein containing Apo A-I and Apo A-II (LpA-I/A-II), and lipoprotein containing Apo A-I but no Apo A-II (LpA-I). To compare the present method with the conventional one which makes use of cyanogen bromide activated agarose, we also tested anti-APO A-I and anti-APO A-II immunoaffinity columns, using a CNBr-activated agarose gel.
収録刊行物
-
- Clinica Chimica Acta
-
Clinica Chimica Acta 179 183-190, 1989-02-01
Elsevier BV