Overexpression of genes of an extreme thermophile Thermus thermophilus, in Escherichia coli cells
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説明
<jats:p>The 3-isopropylmalate dehydrogenase gene from an extreme thermophile, Thermus thermophilus, was not expressed in Escherichia coli unless a palindromic structure around the ribosome binding site was eliminated or a leader open reading frame was introduced into the upstream flanking region of the gene. This report suggests a way to increase the expression of this gene, with a high G+C content, in E. coli.</jats:p>
収録刊行物
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- Journal of Bacteriology
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Journal of Bacteriology 176 2767-2770, 1994-05-01
American Society for Microbiology