Myristoylated -oncogene products are potently detected by the monoclonal antibody to the NH2-terminal myristoyl-Gly-Ser-Ser-Lys -peptide
この論文をさがす
説明
Monoclonal antibodies were raised against a synthetic NH2-terminal myristoyl tetrapeptide (N-myristoyl-Gly-Ser-Ser-Lys) which is characteristic of an NH2-terminal portion of pp60src, the transforming protein of src-oncogene. The antibody reacted with the albumin conjugated with both the N-myristoyl and N-lauroyl-tetrapeptides, but concentrations at which 50% of the immunoreaction was inhibited were 5 pmol for the N-myristoyl and 830 pmol for N-lauroyl tetrapeptidyl albumin. On the other hand, N-palmitoyl tetrapeptidyl and underivatized albumin, and Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys octapeptide had no effects. These results suggest a high affinity of the antibody for an N-myristoyl-Gly-Ser-Ser-Lys moiety. src-Oncogene products in Rous sarcoma virus-transformed cells and human colon carcinoma tumor cells were selectively identified as myristoylated pp60src by immunoprecipitation analyses with the antibody.
収録刊行物
-
- Biochemical and Biophysical Research Communications
-
Biochemical and Biophysical Research Communications 170 657-664, 1990-07-01
Elsevier BV
- Tweet
キーワード
- Blotting, Western
- Molecular Sequence Data
- Antibodies, Monoclonal
- Adenocarcinoma
- Myristic Acid
- Precipitin Tests
- Oncogene Protein pp60(v-src)
- Pancreatic Neoplasms
- Antibody Specificity
- Colonic Neoplasms
- Tumor Cells, Cultured
- Humans
- Amino Acid Sequence
- Multiple Myeloma
- Peptides
- Myristic Acids
- Oligopeptides