Two acid phosphatases in sea urchin eggs and embryos

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Abstract 1. 1. Two types of acid phosphatases from sea urchin eggs and embryos were studied in three Japanese species, Hemicentrotus pulcherrimus, Anthocidaris crassispina and Pseudocentrotus depressus . 2. 2. Acid phosphatase type 1, designated AcP-1, hydrolysed only flavin mononucleotide besides p -nitrophenylphosphate. The activity of AcP-1 was not inhibited by NaF and tartrate. This enzyme showed molecular weight between 14,000 and 16,000 by gel filtration through Sephadex G-75. 3. 3. The higher molecular weight type of acid phosphatase, designated AcP-2, showed relatively high substrate specificity toward ADP and ATP. Molecular weight of AcP-2 ranged from 42,000 to 48,000 by gel filtration through Sephadex G-100. 4. 4. Some properties of AcP-2 from Sphaerechinus granularis embryos are also described.

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