Transcriptional regulation of human N-acetylglucosaminyltransferase IVa gene in trophoblasts

s / Placenta 35 (2014) A1eA112 A50 MTS assay. (3) Migration assay, invasion assay, and adhesion assay were performed. Results: C2GnT was highly expressed in trophoblasts of choriocarcinoma but not in hydatidiform mole. (1) Expression level of C2GnT protein in siRNA transfectants were significantly reduced compared to WT and controls at 24 hours after transfection. (2) MTS assay showed no affection on cell proliferation in Jar after C2GnT siRNA transfection for 72 hours. (3) Suppression of C2GnT expression reduced migration and invasive abilities significantly. The rate of cell attachment to fibronectin, collagen type I, and collagen type IV were decreased by C2GnT knockdown. Conclusion: These findings suggest that C2GnT is involved in regulating invasion of choriocarcinoma and it might be through modifications of the oligosaccharide chains of hCG. P1.127-N. TRANSCRIPTIONAL REGULATION OF HUMAN NACETYLGLUCOSAMINYLTRANSFERASE IVA GENE IN TROPHOBLASTS Yoko Miwa , Eiko Yamamoto , Kanako Shinjo , Kaoru Niimi , Kimihiro Nishino , Yukio Mano , Seiji Sumigama , Tomomi Kotani , Fumitaka Kikkawa a Nagoya University, Nagoya City, Japan; b Toyota Kosei Hospital,