Induction of single-strand DNA breaks with synchrotron radiation in dry Barley nucleus

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Abstract Nuclei isolated from the embryos of dry seeds were exposed to synchrotron radiation (SR) from an electron storage ring (0.3 GeV). Dried nuclei were also gamma-irradiated for comparison. SR of broad wavelength above 115 nm increased the number of 3′-OH termini, which were produced from single-strand DNA breaks in the nucleus. The observed DNA damage is causally-related to the vacuum-u.v. component of SR.

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