A novel pigment protein in the ovary of echinothurid sea urchins, Asthenosoma ijimai and Araeosoma owstoni
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Abstract 1. 1. A protein conjugated with a pigment, which showed a peak of absorbance at 385 nm, was identified and partially purified from the ovary of Asthenosoma ijimai and Araeosoma owstoni by butanol extraction, gel filtration, ion-exchange chromatography and adsorption chromatography. This protein was observed only in ovaries, but not in testes. 2. 2. This protein of A. ijimai showed a molecular weight of 600 kDa on gel filtration. The isoelectric point of the protein was 4.7. 3. 3. The possible presence of this protein was examined by gel filtration chromatography in the ovaries of 11 other species of sea urchins, Glyptocidaris crenularis, Diadema setosum, Temnopleurus hardwicki, Toxopneustes pileolus, Pseudocentrotus depressus, Hemicentrotus pulcherrimus, Strongylocentrotus intermedius, S. nudus, Echinostrephus aciculatus, Anthocidaris crassispina, Echinometra mathaei and Echinocardium cordatum. However, it was not detected.
収録刊行物
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- Comparative Biochemistry and Physiology Part B: Comparative Biochemistry
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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 106 61-66, 1993-09-01
Elsevier BV