The mechanism of sugar uptake in Tetrahymena pyriformis—I. Sugar specificity

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Abstract 1. 1. In Tetrahymena pyriformis , d -glucose was metabolized, but 2-deoxy- d -glucose (2DG) was not metabolized. 2. 2. The curve of uptake velocity vs concentration of the sugars obeyed Langmuir's isotherm, showing the carrier-mediated process. 3. 3. The 2DG uptake system in T. pyriformis showed a high stereo-specificity, i.e. its uptake was inhibited only by d,l -glucose, d -mannose,α,β,-methyl- d -glucoside and 2DG. 4. 4. Counter-flow phenomena of 2DG were observed by the addition of various inhibitory sugars.

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