Isolation of Enterotoxigenic Bacteroides fragilis from Extraintestinal Sites by Cell Culture Assay
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説明
Bacteroides fragilis, the organism most frequently isolated in anaerobic infections, is known to cause intraabdominal, wound, pelvic, perirectal, and bloodstream infections. Recent studies have documented the existence of enterotoxigenic strains of B. fragilis; these strains can cause diarrhea in livestock and humans [1-5]. In 1992 Weikel et al. demonstrated that a human colonic epithelial cell line, HT29/C1, was sensitive to B. fragilis enterotoxin [6]. The cell culture assay developed by these investigators allows us to study enterotoxigenic B. fragilis with much less difficulty than is encountered with the ligated intestinal loop assay [1-5]. In this study, we used the cell culture assay to survey the prevalence of enterotoxin-producing strains of B. fragilis among isolates from extraintestinal sites. Enterotoxigenic B.fragilis strain ATCC (American Type Culture Collection) 43858, nonenterotoxigenic B. fragilis strain ATCC 25285, and 141 clinical strains of B. fragilis isolated from extraintestinal sites in 1987-1989 at the Institute of Anaerobic
収録刊行物
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- Clinical Infectious Diseases
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Clinical Infectious Diseases 20 S141-S141, 1995-06-01
Oxford University Press (OUP)