A Potential Role of Fructose to Modulate Fibroblast Growth and Expression of Connective Tissue Growth Factor In vitro
説明
Aims: Hyperglycemia has been posited to have an effect on the healing process at the cellular level, particularly on fibroblasts that produce extracellular matrix components including collagen. However, the mechanisms underlying this process have yet to be fully elucidated. In addition, excessive intake of fructose-containing drinks has recently been shown to induce hyperglycemia, and fructose induces metabolic changes that may influence normal wound healing. Therefore, we investigated the effect of fructose on the cellular proliferation of cultured fibroblasts. Materials and Methods: The murine fibroblastic cell line NIH3T3 was incubated in the DMEM medium with or without the supplementation of either glucose (0.3, 1.4, or 2.8%) or fructose (1.4 or 2.8%) and was analyzed using microscopy, proliferation assays, and reverse transcriptionpolymerase chain reaction to determine the expression levels of type I collagen and connective
収録刊行物
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- Advances in Research
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Advances in Research 6 1-7, 2016-01-10
Sciencedomain International
