Microdetermination of 2,3-dinor-6-ketoprostaglandin F1α in human urine using gas chromatography—high-resolution selected-ion monitoring
この論文をさがす
説明
The microdetermination of 2,3-dinor-6-ketoprostaglandin F1 alpha (I) in human urine is described. Samples to which the [2H4]-analogue was added as an internal standard were extracted by chromatographic sample preparation using a Bond Elut C18 cartridge and a silica gel column. Conversion of the extracted I into the 1-methyl ester-6-methoxime-9,11,15-trisdimethylisopropylsilyl ether derivative was followed by gas chromatography-high-resolution selected-ion monitoring (GC-HR-SIM). Interfering substances from the urine matrix were eliminated during GC-HR-SIM analysis using a DB-1 column. A good linear response over the range 10 pg-100 ng per tube was demonstrated. Compound I could be detected in the range 26-375 pg/ml of human urine. The proposed method can be applied to the determination of I in human urine.
収録刊行物
-
- Journal of Chromatography B: Biomedical Sciences and Applications
-
Journal of Chromatography B: Biomedical Sciences and Applications 658 11-19, 1994-08-01
Elsevier BV
