Evidence that pretreatment of Escherichia coli cells with N-methyl-N′-nitro-nitrosoguanidine enhances mutability of subsequently infecting phage λ

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説明

The frequency of mutations in λ is significantly higher for host cells of a rec + or recA - strain pretreated with N-methyl-N′-nitro-N-nitrosoguanidine (NG) than for untreated control cells. Direct NG treatment of λDNA-injected host cells results in about tenfold higher mutation frequency in λ than NG treatment of host cells alone. Since mutability of λ is enhanced by UV preirradiation of host cells in the rec + strain but not in the recA - strain, indirect NG mutagenesis is different in mechanism from indirect UV mutagenesis. It is concluded that NG mutagenesis in λ consists of at least two processes: induction of rec +-independent mutagenic activity in the host bacterium and production of rec +-independent mutational damage to intracellular λDNA.

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