Evidence for involvement of dynein-tubulin system in pigment aggregation within tilapia melanophores
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説明
Abstract 1. 1. Using cultured melanophores of the tilapia, Oreochromis niloticus, and Brij 58-permeabilized cells, we investigated the mechanism that drives the aggregation of pigment granules. 2. 2. Although norepinephrine-induced aggregation of melanosomes was not significantly inhibited by pretreatment of cells with cytochalasin B, application of colchicine or vinblastine, both of which are antimitotic poisons, impaired the response of melanophores to the amine in a dose-dependent manner. 3. 3. Indirect immunofluorescence staining showed that the radial arrangement of microtubules within tilapia melanophores disappeared after melanophores had been exposed to colchicine or vinblastine. A normal distribution of microtubules was observed in cytochalasin-treated cells that responded to norepinephrine by pigment aggregation. 4. 4. Erythro-9-[3-(2-hydroxynonyl)]adenine (EHNA) and vanadate, inhibitors of dynein ATPase, were also effective in blocking the norepinephrine-induced aggregation of melanosomes. 5. 5. From these results, we conclude that cytoplasmic microtubules are essential for the centripetal translocation of melanosomes, and that the motive force involved in the process may be generated by interactions between dynein and tubulin.
収録刊行物
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- Comparative Biochemistry and Physiology Part A: Physiology
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Comparative Biochemistry and Physiology Part A: Physiology 96 517-523, 1990-01-01
Elsevier BV