Binding Constant of V<sup>IV</sup>O to Transferrin

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<jats:title>Abstract</jats:title><jats:p>The interactions of bis‐V<jats:sup>IV</jats:sup>O‐1,2‐dimethyl‐3‐hydroxy‐4(1<jats:italic>H</jats:italic>)‐pyridinone (DHP) with apotransferrin (apoTF) were followed by means of electron paramagnetic resonance spectroscopy (EPR) at room temperature and at liquid nitrogen temperature. Partial ligand displacement between DHP and apoTF was observed, and the reaction was used to determine the binding constant of V<jats:sup>IV</jats:sup>O to apoTF. A value of log <jats:italic>K</jats:italic> = 14.3 ± 0.6 was obtained for the binding of the first V<jats:sup>IV</jats:sup>O to apoTF. In order to confirm the validity of the binding constant, UV spectroscopy was used to monitor the displacement reaction between V<jats:sup>IV</jats:sup>O–apoTF and nitrilotriacetic acid (NTA). The difference between the binding constants obtained by the two different methods (ca. one log unit) was explained in terms of the possible ternary complex formation between V<jats:sup>IV</jats:sup>O–apoTF and the displacing ligand. The binding constants obtained exhibit a reasonably good agreement with the linear free energy relationship (LFER) estimation. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)</jats:p>

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詳細情報 詳細情報について

  • CRID
    1871146592690859904
  • DOI
    10.1002/ejic.200600314
  • ISSN
    10990682
    14341948
  • データソース種別
    • OpenAIRE

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