483—Monitoring cell potential of Escherichia coli with use of an electrode sensitive to tetraphenyl phosphonium

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Abstract An electrode sensitive to tetraphenyl phosphonium cation (TPP+) was applied to the determination of the membrane potential of E. coli (strain B, IAM 1268). This strain did not require EDTA (ethylenediamine tetraacetic acid)-treatment for accumulation of TPP+. As reported previously, some part of the TPP+-uptake was due to the binding to cell envelopes. The time course of TPP+-uptake by cells on addition of sugars was analysed, and the result indicated that the additional binding of TPP+ no longer occured. The change in the membrane potential on addition of various sugars was evaluated.

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