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説明
Abstract The identification and intracellular localization of DNA-degrading enzymes released from Escherichia coli by three treatments affecting the cell surface were investigated and the following results were obtained. 1. 1. Three DNA-degrading enzymes, i.e. endonuclease I (deoxyribonucleate oligonucleotide hydrolase, EC 3.1.4.5) exonuclease II and another unidentified exonuclease, are released by various treatments but exonuclease I is not released. These results suggest that the three former deoxyribonucleases are localized near the cell surface of bacteria. 2. 2. The releasabilities of the three deoxyribonucleases differ depending on the treatment used. This suggests that the three deoxyribonucleases are in different states on the bacterial cell surface. 3. 3. The releasabilities of each deoxyribonuclease varied with the growth phase. It is to be noted that the location of DNA polymerase-associated exonuclease II changed with change in the physiological state. The physiological roles of these enzymes are discussed in relation to these phenomena.
収録刊行物
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- Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis 155 98-106, 1968-01-01
Elsevier BV