Suppression of lipid synthesis in cultured aortic cells by laminaran sulfate

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Summary (1)Sodium [1- 14 C]acetate was sextensively incorporated into the lipid fractions of chick aortic cells in secondary cultures maintained as monolayers, indicating a linear relation between the incorporation rate and the incubation time over 8 h. (2)When laminaran sulfate was added to this culture system, at concentrations between 2.5 and 40 μg/ml culture medium for 24–72 h, it depressed lipid synthesis in aortic cells, as measured by [ 14 C]acetate incorporation rates into the lipid fraction. (3)Inhibition of lipid synthesis depended on the concentration of laminaran sulfate and its incubation time; inhibition was greater in the neutral fat and free sterol fractions than in the phospholipid fraction.

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