Elucidation of iridodial formation mechanism - partial purification and characterization of the novel monoterpene cyclase from cell suspension cultures

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Abstract From the incubation system of 10-hydroxygeraniol and crude enzyme extracts from Rauwolfia serpentina cell suspension cultures, 10-oxogeraniol, 10-hydroxygeranial and 10-oxogeranial were isolated. A precursory role in the biosynthesis of iridodial has been demonstrated for these three compounds. This fact unequivocally substantiates our previous claim for the biosynthesis of iridodial. In addition, a novel monoterpene cyclase catalyzing the iridodial formation was partially purified and characterized.

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