Diffication of high affinity fatty acid receptors in rat myocardial sarcolemmal membranes

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<jats:title>Abstract</jats:title><jats:p>High affinity receptors for fatty acid were purified from rat cardiac sarcolemmal membrane using gel filtration, DEAE‐cellulose chromatography and affinity chromatography. The purified protein was homogeneous on polyacrylamide gel electrophoresis with the molecular weight of 60 kDa. Binding studies revealed the presence of a single class of high affinity binding sites with an apparent dissociation constant of 1.0 μM and a maximal binding capacity of 12.1 pmol/μg protein.</jats:p>

収録刊行物

  • Lipids

    Lipids 22 544-546, 1987-07-01

    Wiley

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