書誌事項
- タイトル別名
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- A bifunctional enzyme responsible for both poly(A)‐polymerizing and poly(A)‐hydrolyzing activities
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説明
<jats:p>Poly(A)‐specific ribonuclease was co‐purified with poly(A) polymerase from <jats:italic>Vigna unguiculata</jats:italic> seedlings. Both activities were separated into two forms (enzymes I and II) by a final hydrophobic column chromatography. The enzyme I preparation, which was homogeneous as examined by SDS/PAGE, had both poly(A) polymerase and poly(A)‐specific ribonuclease activities. The antibody raised to the enzyme I preparation precipitated both enzyme activities. These indicate that a single polypeptide (<jats:italic>M</jats:italic><jats:sub>r</jats:sub> 63 000) is responsible for both poly(A)‐polymerizing and poly(A)‐hydrolyzing activities. The poly(A)‐specific ribonuclease was a 3′‐exonuclease specific to single‐stranded poly(A), forming 5′AMP as the sole reaction product. The hydrolytic activity required either Mn<jats:sup>2+</jats:sup> or Mg<jats:sup>2+</jats:sup> with different optimum concentrations, whereas the polymerizing activity required Mn<jats:sup>2+</jats:sup> but not Mg<jats:sup>2+</jats:sup>. ATP and PP<jats:sub>i</jats:sub> had little or no effect on the poly(A)‐specific ribonuclease activity.</jats:p>
収録刊行物
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- European Journal of Biochemistry
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European Journal of Biochemistry 186 591-596, 1989-12-01
Wiley
