Super-duper chemiluminescent proteins applicable to wide range of bioimaging

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説明

We report five new spectral variants of bright luminescent protein made by concatenation of the brightest luciferase, NanoLuc, with various color hues of fluorescent proteins. These proteins, which we call enhanced Nano-lanterns (eNLs), allow five-color live-cell imaging without external light illumination as well as detection of single molecules. Furthermore, eNL-based Ca2+ indicators could be used to image long-term Ca2+ dynamics in iPS-derived cardiomyocytes.

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詳細情報 詳細情報について

  • CRID
    1871709542408591360
  • DOI
    10.1117/12.2249770
  • ISSN
    0277786X
  • データソース種別
    • OpenAIRE

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